An ESIstream package can contain several TCL scripts. The number 16, 32 or 64 indicates the serialization or deserialization width used to configure the User data width parameter value of the Xilinx Gigabit transceiver IP, shown on the picture below.
The selection of user data width (16, 32 or 64) is a trade-off between minimum link latency, minimum logic resources, and frames rate in the FPGA.